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99% of experimental failures stem from this step! Do you really know how to maintain your CO2 incubator?

 Date:2025-12-03 11:14:51

Many researchers have experienced the frustrating moments when their cells appear lethargic or even inexplicably die off. The first instinct is often to check the serum or techniques used, but they overlook the critical environment—the CO2 incubator—where the cells thrive.

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As a core device for simulating the in vivo environment, if the incubator is not cleaned properly on a daily basis, its interior can become a breeding ground for contaminants such as bacteria, fungi, mycoplasma, and even aerosols. These invisible "silent killers" can subtly alter cellular metabolism, rendering countless days of experimental data meaningless.

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To safeguard scientific research achievements

To safeguard scientific research achievements

Must master the combination of "hardcore disinfection+fine maintenance"!

Reject fake cleanliness! Choose the right "Mieba" level disinfection

Faced with stubborn bacterial spores and fungi, ordinary wiping often treats the symptoms rather than the root cause. According to the sterilization principle, please take your seat accordingly:

Surface cleaning (basic version): 75% alcohol

Although it is easy to operate, it can only perform surface disinfection and cannot completely kill stubborn spores, and it is strictly prohibited to spray on the sensor.

Physical Assistance (Advanced Version): HEPA Filter&UV Lamp

It can effectively filter particulate bacteria in the air or carry out radiation sterilization, but there are blind spots for irradiation, and consumables need to be replaced regularly.

High temperature sterilization (ultimate version): 180 ℃ dry heat is currently recognized as the most thorough sterilization method! Compared to the time-consuming wet heat disinfection, a high temperature of 180 ℃ can penetrate blind spots and completely kill all microorganisms, including high-temperature resistant spores, giving precious cells a truly sterile "home".

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high-temperature sterilization

Ready to use! Daily maintenance "nanny level" checklist

 

Disinfection alone is not enough, standardized daily maintenance is the key to preventing pollution from returning. Please forward this list based on original factory standards to our laboratory colleagues:

 

The Iron Law of 'Water'

 

What water should be added? It is not recommended to use tap water or deionized water. It is recommended to use sterile distilled water.

 

How long does it take to change? It is recommended to replace the water in the water tray once a week, as this is the bottom line to prevent pollution from breeding.

 

Cleaning frequency and taboos

 

■ Once a month: Use 75% alcohol to wipe the inner container, partition, and accessories.

 

Important reminder: When wiping, absolutely avoid CO2 sensors and temperature probes to prevent corrosion damage; If using a water jacket incubator, it is necessary to regularly monitor the water level of the water jacket layer.

 

Gas supply and filter element

 

Gas cylinder management: Maintain pressure between 0.08MPa and 0.1MPa. Never use the gas cylinder until it is completely empty before replacing it, as low air pressure can affect the intake and cause abnormal concentration or even cultivation failure.

 

■ Shield of Life: It is recommended to replace the intake and HEPA filters inside the box every 12 months, as this is the final hurdle in blocking the entry of external bacteria.

 

Discontinued and idle

 

Long holiday treatment: If cells are not cultured for a long time, the water tray must be emptied and the gas and power sources must be turned off.

 

Key step: Open the box door and ventilate for at least 24 hours to ensure thorough drying inside. A damp and enclosed environment is a paradise for mold!

 

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Clean and tidy incubator body after maintenance

 

Almighty Warrior


HF180 Three Gas Incubator

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This is to tackle cutting-edge scientific research challenges


The ultimate weapon born!

 

■ Gas sleeve heating, opening speed


8-inch touchscreen operation, dynamic monitoring and display of data


180 ℃ high temperature dry heat sterilization, short sterilization time


■ Built in HEPA filter, the box reaches level 100 within 5 minutes after the door is closed


■ Forward tilting chassis reservoir design, fast speed recovery speed


■ Equipped with high-temperature sterilization door lock design, safe and reliable


■ Standard fast drainage design, easy daily maintenance


■ With multiple data output functions and Biolab central monitoring system


Stackable placement, saving laboratory space

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Sharp tools make good work.

Treat the incubator with the same tenderness as you treat your cells. Take these steps well, bid farewell to pollution anxiety, and let your cells win at the starting line!

 

Heal Force Intelligent Manufacturing is willing to protect every effort you make for scientific research with super powerful professional equipment!

 

For more details, please feel free to call us for consultation


400 820 6276

[Disclaimer] Some of the information in this article is sourced from the internet. If there is any infringement, please contact us for deletion

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